Reconstruction of unstained embryos for the Edinburgh Mouse Atlas

The following movies show two embryos (a TS19 and TS20) at the various stages during the process. The embryos were fixed in Bouin's solution, which increases their opacity, and the first step therefore was to make surface movies (left). Next the embryos were incubated in BABB to make them almost completely transparent, and scanned again to make transmission movies (middle). The projection data represented by these movies was then fed into the back-projection algorithm to generate full 3-D reconstructions (right).